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探讨解淀粉芽孢杆菌15-1-1菌株液体发酵条件,为提高其活性次级代谢产物的产量提供支持。通过单因素试验和正交试验,对该菌株的最适发酵培养基成分及发酵条件进行优化,结果表明:15-1-1菌株培养基各组分的最佳配比为:葡萄糖2.0%、蛋白胨0.5%、酵母浸膏1.0%、氯化钠0.5%。最佳发酵培养条件为:初始p H值7.0,培养温度32℃,培养时间36 h,250 m L三角瓶通气量40 m L,接种量体积分数5%。
The liquid fermentation conditions of Bacillus amyloliquefaciens 15-1-1 were explored to provide support for increasing the production of secondary metabolites. The optimum fermentation medium composition and fermentation conditions of the strain were optimized by single factor experiment and orthogonal experiment. The results showed that the optimal ratio of each component of 15-1-1 strain medium was 2.0% glucose, 0.5% peptone, yeast extract 1.0%, sodium chloride 0.5%. The optimal fermentation conditions were as follows: initial pH 7.0, incubation temperature 32 ℃, incubation time 36 h, volume of 250 mL flask 40 mL, volume of inoculum 5%.