收集石腊包埋组织标本膀胱癌８０例、肺癌６９例、大肠癌３７例，对有转移者同时收集其转移灶标本，应用免疫组织化学法检测ＮＤＰＫ／ｎｍ２３－Ｈ１的表达情况。结果显示：ＮＤＰＫ／ｎｍ２３－Ｈ１在膀胱癌、肺癌和大肠癌中表达的阳性率分别为６４％、８０％和９４％；其表达水平在膀胱癌、大肠癌与分化程度有关，随分化程度降低而显著降低（Ｐ＜００５）；在肺癌与组织学类型有关，以肺鳞癌中最高，其次为腺癌和小细胞癌（Ｐ＜００５）；在大肠癌与淋巴结转移有关，伴淋巴结转移者其原发灶中显著降低（Ｐ＜００１）；对原发灶和转移灶的配对分析，未发现相关性。提示ＮＤＰＫ／ｎｍ２３－Ｈ１表达水平是一个与肿瘤恶性行为相关的因素，但其临床意义存在组织学差异。 Paraffin-embedded tissue specimens were collected from 80 cases of bladder cancer, 69 cases of lung cancer, and 37 cases of colorectal cancer. The metastatic specimens were collected from metastases and the expression of NDPK/nm23-H1 was detected by immunohistochemistry. The results showed that the positive rates of NDPK/nm23-H1 expression in bladder, lung, and colorectal cancers were 64%, 80%, and 94%, respectively; the expression levels of NDPK/nm23-H1 were related to the degree of differentiation in bladder cancer, colorectal cancer, and the degree of differentiation decreased. Significantly lower (P<005); in lung cancer and histological type, the highest in lung squamous cell carcinoma, followed by adenocarcinoma and small cell carcinoma (P<005); in colorectal cancer and lymph node metastasis, In patients with lymph node metastasis, their primary lesions were significantly reduced (P<001); no correlation was found for the paired analysis of primary and metastatic lesions. It is suggested that the expression level of NDPK/nm23-H1 is a factor related to the malignant behavior of tumors, but its clinical significance exists histological differences.