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研究目的:深入分析两个红肉桃品种果实发育期花色素苷合成差异的分子机理,为红肉桃品种的遗传改良与利用提供理论依据。创新要点:阐明两个红肉桃品种果实发育期花色素苷生物的合成与相关基因的时空表达规律,探讨造成两个品种花色素苷积累差异的内在分子机制。研究方法:应用高效液相色谱(HPLC)和逆转录聚合酶链式反应(RT-PCR)分别鉴定两个红肉桃品种花色素苷的积累规律与合成相关基因的表达水平,进一步探讨两者花色素苷合成的内在分子调控机理。重要结论:两个红肉桃品种果实发育期花色素苷合成与PpCHS表达水平密切相关,同时PpPAL在白肉桃品种果实发育期的表达水平低于红肉桃品种,推测这两种基因可能是限制白肉桃品种果实中花色素苷合成的因素之一。
Research purposes: In-depth analysis of the molecular mechanism of anthocyanin biosynthesis in fruit development of two red peach varieties provides theoretical basis for the genetic improvement and utilization of red peach varieties. Innovative points: To elucidate the biosynthesis of anthocyanins and the temporal and spatial expression of related genes during the fruit development of two red peach cultivars, and to explore the underlying molecular mechanism that led to the differences in the accumulation of anthocyanins between the two cultivars. Methods: HPLC and reverse transcription polymerase chain reaction (PCR) were used to identify the accumulation of anthocyanins and the expression of related genes in two red peach cultivars Internal Molecular Mechanism of Anthocyanin Synthesis. Important conclusion: Anthocyanin biosynthesis is closely related to the expression of PpCHS during the fruit development of two red peach cultivars, and the expression level of PpPAL is lower than that of red peach cultivars at the fruit development stage. It is speculated that these two genes may be restricted One of the factors of anthocyanin synthesis in white peach varieties.