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采用“扭体法”和“热板法”研究了“芩红乳房灌注剂”的镇痛作用,用二甲苯致小鼠耳廓肿胀试验研究了该药的抗炎作用,并通过急性毒性试验对其安全性进行评价,测出了该药的最大给药量与最大耐受倍数。镇痛试验结果表明,“芩红乳房灌注剂”高剂量组、中剂量组、低剂量组和安痛定组的扭体反应次数分别为28.8±2.3,29.6±3.1,38.8±2.3和13.9±2.6,均显著低于生理盐水组(P<0.01或P<0.05);“热板法”试验在60 min和90 min时高剂量、中剂量与低剂量组、对照组相比差异显著(P<0.05),且在给药120 min低剂量的芩红乳房灌注剂和生理盐水组差异不显著(P>0.05),而高、中剂量与生理盐水组相比差异显著(0.010.05),但是仍表现出11.11%的肿胀抑制率。急性毒性试验未测出药物的半数致死量(LD_(50)),最大给药量为80 g/kg,最大耐受倍数为临床奶牛拟用剂量的2 000倍。结果显示,芩红乳房灌注剂具有良好的镇痛、抗炎作用且应用安全。
The analgesic effect of “Scutella breast breast perfusion agent ” was investigated by “writhing method ” and “hot plate method ”. The anti-inflammatory effect of the drug was studied by the auricle swelling test in mice induced by xylene , And its safety was evaluated by acute toxicity test. The maximum dose and the maximum tolerable dose of the drug were measured. The results of analgesic test showed that the number of writhing reactions in the “high-dose group, middle-dose group, low-dose group and Antongding group were 28.8 ± 2.3,29.6 ± 3.1,38.8 ± 2.3 and 13.9 ± 2.6, all of which were significantly lower than that of the saline group (P <0.01 or P <0.05). Compared with the control group, the ”hot plate test" at 60 min and 90 min (P <0.05), and there was no significant difference between the control group and the saline group (P> 0.05)
0.05), but still showed 11.11% swelling inhibition rate. Acute toxicity test did not detect the median lethal dose of drug (LD 50), the maximum dose of 80 g / kg, the maximum tolerated multiple clinical cow dose of 2 000 times. The results show that Qin Hong breast perfusion agent has good analgesic, anti-inflammatory effect and application safety.