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目的通过建立肺炎支原体(MP)感染BALB/c小鼠的模型,探讨克拉霉素对肺炎支原体感染小鼠IL-10表达的影响。方法 60只昆明小鼠随机分为对照组、MP感染组及克拉霉素治疗组,每组20只,建立小鼠肺炎支原体感染模型,克拉霉素治疗组用克拉霉素治疗5 d。HE染色观察病理组织学变化;Western blot方法检测各组小鼠肺组织IL-10的表达;RT-PCR方法检测各组小鼠肺组织IL-10m RNA的表达水平。结果 MP感染后,小鼠肺组织呈间质性炎性改变,肺泡间隔增宽,大量淋巴细胞和巨噬细胞浸润在支气管、血管周围,肺泡内有渗出。相比于对照组,MP感染组小鼠肺组织IL-10蛋白和m RNA表达水平明显降低(<0.01);与MP感染组相比,克拉霉素治疗组小鼠肺组织IL-10蛋白和m RNA表达水平明显升高(<0.01)。结论克拉霉素可显著上调肺炎支原体感染小鼠肺组织IL-10水平。
Objective To investigate the effect of clarithromycin on IL-10 expression in mice infected with Mycoplasma pneumoniae by establishing a model of Mycoplasma pneumoniae (MP) infection in BALB / c mice. Methods Sixty Kunming mice were randomly divided into control group, MP infection group and clarithromycin treatment group, with 20 mice in each group. Mycoplasma pneumoniae infection model was established in mice and clarithromycin treatment for 5 days in clarithromycin treatment group. HE staining was used to observe the histopathological changes. The expression of IL-10 in lung tissue was detected by Western blot and the expression of IL-10mRNA in lung tissues was detected by RT-PCR. Results After MP infection, the lung tissues of mice exhibited interstitial inflammatory changes, widened alveolar septum, infiltration of large numbers of lymphocytes and macrophages in the bronchi, perivascular and alveolar spaces. Compared with the control group, the expression of IL-10 protein and m RNA in the lungs of MP-infected mice was significantly decreased (P <0.01). Compared with the MP-infected mice, the levels of IL-10 protein and m RNA expression was significantly increased (<0.01). Conclusion Clarithromycin can significantly up-regulate the level of IL-10 in lung tissue of mice infected with Mycoplasma pneumoniae.