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目的 探讨T淋巴细胞亚群、可溶性白介素2受体和肿瘤坏死因子α在梅毒发病机制中的作用。方法 应用流式细胞仪和双抗体夹心ELISA法对86例梅毒患者T淋巴细胞亚群（CD3+、CD4+、CD8+）、可溶性白介素2受体（sIL-2R）及肿瘤坏死因子α（TNF-α）水平进行检测,并对其相关性进行分析。结果 梅毒患者CD4+及CD4+/CD8+比值明显低于正常人对照组（P<0.01）,CD8+显著高于正常人对照组（P<0.05）,而CD3+与正常人对照组无显著性差异（P>0.05）;活动期梅毒CD4+/CD8+比值低于恢复期（P<0.05）。活动期梅毒及非活动期梅毒患者血清sIL-2R水平均明显增高（P<0.01）,且活动期高于非活动期（P<0.01）。活动期梅毒患者血清TNF-α水平高于非活动期及正常对照组（P<0.01）,且活动期高于非活动期（P<0.01）。活动期梅毒患者 CD4+、CD8+与 sIL-2R和 TNF-α水平呈正、负相关。结论 本研究提示梅毒患者存在细胞免疫功能抑制现象,且 T淋巴细胞功能的紊乱和TNF-α及sIL-2R水平的改变与梅毒的发病有密切关系。
Objective To investigate the role of T lymphocyte subsets, soluble interleukin 2 receptor and tumor necrosis factor α in the pathogenesis of syphilis. Methods T-lymphocyte subsets (CD3 +, CD4 +, CD8 +), soluble interleukin 2 receptor (sIL-2R) and tumor necrosis factor-α (TNF- α) in 86 patients with syphilis were detected by flow cytometry and double antibody sandwich ELISA. Level test, and its correlation analysis. Results The ratio of CD4 + and CD4 + / CD8 + of syphilis patients was significantly lower than that of normal controls (P <0.01), while that of CD8 + was significantly higher than that of normal controls (P <0.05). There was no significant difference between CD3 + 0.05). The ratio of CD4 + / CD8 + in active syphilis was lower than that in recovery (P <0.05). Serum sIL-2R levels were significantly higher in active syphilis and inactive syphilis patients (P <0.01), and the activity was higher than inactive (P <0.01). Serum levels of TNF-α in patients with active syphilis were significantly higher than those in non-active and normal controls (P <0.01), and the activity was higher than that in inactive (P <0.01). Active syphilis patients CD4 +, CD8 + and sIL-2R and TNF-α levels were positive and negative correlation. Conclusions This study suggests that there is suppression of cellular immune function in patients with syphilis, and the disorder of T lymphocyte function and the change of TNF-α and sIL-2R levels are closely related to the pathogenesis of syphilis.