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用循环伏安(CV)法,在玻碳电极上电沉积制备食用靛蓝(IC)修饰电极,研究了IC活性膜的电化学性质。在pH 2.30的磷酸盐缓冲液中,IC在修饰电极上的电化学行为符合可逆过程的特征,且电子传递过程受表面过程控制。求得IC活性膜在电极表面的电子传递系数为0.57,电荷转移速率常数为1.97s-1。研究了抗坏血酸(AA)和尿酸(UA)在该电极上的电化学行为,该电极显著降低了AA和UA的氧化过电位,对两者有较好的电催化活性。差分脉冲伏安(DPV)实验表明AA、UA氧化峰电流与浓度分别在1.0×10-3～1.0×10-2mol/L和5.0×10-5～8.0×10-4mol/L范围内呈线性关系,而且AA和UA在IC修饰电极上氧化峰电位差ΔEp为0.23V,可实现两种物质的同时检测。
Electrochemical deposition of edible indigo (IC) was electrodeposited on glassy carbon electrode by cyclic voltammetry (CV) method. The electrochemical properties of IC active film were studied. In pH 2.30 phosphate buffer, the electrochemical behavior of IC at the modified electrode is in accordance with the characteristics of the reversible process, and the electron transport process is controlled by the surface process. The electron transfer coefficient of the IC active membrane at the electrode surface was found to be 0.57 and the charge transfer rate constant was 1.97s-1. The electrochemical behavior of ascorbic acid (AA) and uric acid (UA) on the electrode was studied. The electrode significantly reduced the overpotential of AA and UA, and had good electrocatalytic activity for both. Differential pulse voltammetry (DPV) experiments showed that the oxidation peak currents of AA and UA were linear in the range of 1.0 × 10-3 ~ 1.0 × 10-2 mol / L and 5.0 × 10-5 ~ 8.0 × 10-4 mol / L, respectively Relationship, and AA and UA modified electrode in the IC oxidation peak potential difference ΔEp 0.23V, enabling the simultaneous detection of two substances.