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目的:探讨牙髓卟啉单胞菌(P.e)脂多糖(LPS)对小鼠成骨细胞株MC3T3-E1细胞ERK1/2和p38表达的影响。方法:用10μg/mL的P.e-LPS分别作用于成骨细胞0、5、15、30、60、180 min,应用蛋白质印迹技术检测成骨细胞内磷酸化ERK1/2和p38表达水平的变化。采用SPSS11.0软件包对结果进行单因素方差分析和Dunnett t检验。结果:10μg/mL的P.e-LPS刺激后,成骨细胞内p38迅速被活化,5～30 min为激活高峰(P<0.01),60 min后基本恢复至正常水平;ERK1/2磷酸化水平在P.e-LPS刺激5 min后明显增加,15 min后磷酸化水平最高(P<0.01),30 min后磷酸化水平下降。结论:P.e-LPS可诱导成骨细胞MC3T3-E1的p38和ERK1/2蛋白表达增强,Pe-LPS可能通过p38和ERK1/2对成骨细胞发挥作用。
Objective: To investigate the effect of P.e lipopolysaccharide (LPS) on the expression of ERK1 / 2 and p38 in mouse osteoblastic cell line MC3T3-E1. METHODS: Osteoblasts were treated with 10 μg / mL P.e-LPS for 0, 5, 15, 30, 60 and 180 min, respectively. Western blotting was used to detect the expression of phosphorylated ERK1 / 2 and p38 in osteoblasts. One-way ANOVA and Dunnett t test were performed on SPSS 11.0 software package. Results: After stimulation with Pe-LPS at 10μg / mL, p38 was rapidly activated in osteoblasts, peaked at 5-30 min (P <0.01), and returned to normal levels after 60 min. The phosphorylation level of ERK1 / 2 Pe-LPS stimulation increased significantly after 5 min, and reached the peak at 15 min (P <0.01). The phosphorylation level decreased after 30 min. CONCLUSION: P38 and ERK1 / 2 protein expression of osteoblasts MC3T3-E1 can be induced by P.e-LPS, and Pe-LPS may play an important role on osteoblasts through p38 and ERK1 / 2.