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目的探讨儿科铜绿假单胞菌(PA)的耐药性及产超广谱β-内酰胺酶(ESBLs)特点。方法运用全自动微生物分析仪鉴定细菌;药物敏感性试验采用琼脂稀释法;采用ESBLs检测试剂盒检测ESBLs;统计PA的标本类型、各类标本的多重耐药及产ESBLs情况,采用χ2检验分析数据。结果共分离114株PA,其中多重耐药菌占28.07%(32株)。产ESBLs菌株占34.21%(39株),其中为多重耐药菌占76.92%(30株)。环丙沙星的耐药率最低(15.79%),而复方新诺明最高(79.82%)。环丙沙星和头孢他啶在ESBLs阳性组的耐药率明显高于ESBLs阴性组(P<0.05)。来源于痰液的PA最为常见(71.05%)。结论儿科PA的耐药形势严峻,可感染患儿多个部位。PA耐药性与ESBLs关系密切。
Objective To investigate the drug resistance of Pediatric Pseudomonas aeruginosa (PA) and the characteristics of producing extended-spectrum β-lactamases (ESBLs). Methods The bacteria were identified by automatic microbiological analyzer. The drug sensitivity test was performed by agar dilution method. ESBLs detection kit was used to detect ESBLs. The types of PA samples, multidrug resistance and ESBLs production of all kinds of samples were analyzed. . Results A total of 114 PA isolates were isolated, of which 28.07% (32 strains) were multi-drug resistant. ESBLs-producing strains accounted for 34.21% (39 strains), of which 76.92% were multi-drug resistant strains (30 strains). Ciprofloxacin the lowest rate of drug resistance (15.79%), while the highest cotrimoxazole (79.82%). The drug resistance rates of ciprofloxacin and ceftazidime in ESBLs positive group were significantly higher than those in ESBLs negative group (P <0.05). PA from sputum is the most common (71.05%). Conclusion PA pediatric drug resistance situation is severe, can affect multiple parts of children. PA resistance is closely related to ESBLs.