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目的建立美花石斛HPLC特征图谱分析方法,研究美花石斛及其水煎液指纹图谱的相关性。方法采用Kromasil 100-5 C_(18)色谱柱;流动相为乙腈-0.2%磷酸溶液,梯度洗脱;检测波长为280 nm;柱温为35℃;流速为0.8 mL·min~(-1)。结果 16批美花石斛药材样品,标示出22个特征共有峰,相似度为0.854~0.969;10批美花石斛水煎液样品,标示出药材中的16个共有峰,相似度均在0.909以上。经对照品保留时间定位及特征峰紫外光谱分析,鉴别了2个特征峰(峰14为柚皮素、峰16为石斛酚)。每批药材与其水煎液间的相似度为0.815~0.910。结论方法准确可靠稳定,重复性好,为美花石斛质量控制和物质基础研究提供参考。
OBJECTIVE: To establish a method for HPLC characterization of Dendrobium chrysanthum and study the correlation between fingerprints of Dendrobium chrysanthum and its decoction. Methods The Kromasil 100-5 C 18 column was used. The mobile phase consisted of acetonitrile-0.2% phosphoric acid solution with gradient elution. The detection wavelength was 280 nm. The column temperature was 35 ℃ and the flow rate was 0.8 mL · min -1. . Results 16 samples of Dendrobium officinale showed 22 characteristic common peaks with similarities of 0.854-0.969. Ten batches of samples of Dendrobium officinale decoction showed 16 common peaks in the medicinal materials with the similarity values above 0.909 . The retention time of reference substance and the characteristic peak UV spectrum analysis identified two characteristic peaks (peak 14 is naringenin and peak 16 is gigantol). The similarity between each batch of herbs and its decoction is 0.815 ~ 0.910. Conclusion The method is accurate, reliable and stable with good repeatability and provides a reference for the quality control and material basis of Dendrobium officinale.