血液中微量元素水平能够直接反映儿童的营养状况，我们建立了一滴耳垂血—火焰原子吸收分析方法，对２６９名学龄前儿童锌、铜、铁和钙的含量进行了测定和分析。用一滴耳垂血测定锌、铜、铁和钙四种元素的方法具有简易、快速、准确、采血量小、损伤和痛苦少、患者（尤其是儿童）易于接受等优点；因此适用大批血液试样分析和流行病学调查，有利于推广应用。 Trace elements levels in the blood can directly reflect children’s nutritional status. We established a drop of earlobe blood - flame atomic absorption spectrometry to determine and analyze the contents of zinc, copper, iron and calcium in 269 preschool children. The method of determining the four elements of zinc, copper, iron and calcium with a drop of earlobe blood is simple, rapid, accurate, small in blood volume, less damaging and painful, and easy to accept by the patient (especially children). Therefore, a large number of blood samples Analysis and epidemiological investigation is conducive to the promotion and application.