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培育抗病品种是大豆胞囊线虫(Soybean Cyst Nematode,SCN)病经济、有效的防治方法。利用130个SSR标记对26份抗SCN4号生理小种(SCN4)新品系和15份感病品系进行基因型分析,旨在明确抗病品系与SCN4抗性相关联的SSR标记,提出抗性基因分子标记鉴定方法,以提高抗病品系在育种中的利用效率。研究表明,Hartwig与晋品系亲本具有不同的SCN4抗病基因,其遗传相似系数为0.362。与抗性显著关联的22个SSR位点分布在11个连锁群(LG),推测LGD1b上分布的SSR标记附近存在1个新的SCN4抗病基因;而Satt684、Sat_230、Sat_222、Satt615和Satt231位点,来自亲本Hartwig等位基因与抗病相关联,而来自晋品系的等位基因与感病相关联,在Sat_400、Satt329和Satt557等其他17个SSR位点,来自Hartwig等位基因与感病相关联,来自晋品系亲本的等位基因与抗病相关联。利用非连锁不平衡SSR标记Satt684和Sat_400可对供试品系进行有效的抗性辅助选择。
Breeding of resistant varieties is an economical and effective method for the prevention and treatment of Soybean Cyst Nematode (SCN). A total of 26 SSR-resistant SCN4 lines and 15 susceptible lines were genotyped using 130 SSR markers to identify SSR markers associated with SCN4 resistance in resistant lines and to propose resistance genes Molecular marker identification methods to improve the efficiency of the use of resistant strains in breeding. Studies have shown that Hartwig and Jin strains have different SCN4 resistance genes with a genetic similarity coefficient of 0.362. The 22 SSR loci significantly associated with resistance were located in 11 linkage groups (LG), suggesting that there was one new SCN4 resistance gene near the SSR markers distributed on LGD1b, whereas Satt684, Sat_230, Sat222, Satt615 and Satt231 Point, the Hartwig allele from the parents was associated with disease resistance, while the alleles from the Jin line were associated with susceptibility. In the other 17 SSR loci such as Sat_400, Satt329 and Satt557, the Hartwig allele was associated with susceptibility , The alleles from the parents of the Jin strains were associated with disease resistance. The non-linkage disequilibrium SSR markers Satt684 and Sat_400 could be used as effective resistance aids to the tested lines.