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最近报道,用仅含不同特性的合成肽的共聚体免疫小鼠,在无任何蛋白或多肽载体的情况下,仍能产生生物活性抗体,作者采用4种合成肽:链球菌肽S-34、白喉杆菌肽D、疟原虫肽PK26和乙型肝炎病毒肽H(99-121),前3种肽已有人证明能诱导生物活性抗体。用4种肽的共聚体或同聚物PK26或S-34免疫小鼠。采用戊二醛可达到不同肽的异分子聚合,即12.6mgS-34、10.1mgD、12.1mgH以及14.2mgPK26在9.8ml0.1M碳酸氢钠溶液中混合1小时,戊二醛加至最后浓度为2.63mM,在室温内连续搅拌,培育2周后以PBS彻底透析,重新获取的肽总量可达90%。
Recently, it has been reported that immunization of mice with an interpolymer of synthetic peptides containing only different properties still produces bioactive antibodies in the absence of any protein or polypeptide carrier. The authors used four synthetic peptides, streptococcal S-34, Diphtheria bacili peptide D, Plasmodium pylori PK26 and Hepatitis B virus peptide H (99-121), the first three peptides have been shown to induce bioactive antibodies. Mice were immunized with intermutals or homopolymers PK26 or S-34 of 4 peptides. The use of glutaraldehyde allows for heterogeneous polymerization of different peptides, i.e. 12.6 mg S-34, 10.1 mg D, 12.1 mg H and 14.2 mg PK26 in 9.8 ml of 0.1 M sodium bicarbonate for 1 hour and glutaraldehyde to a final concentration of 2.63 mM. The mixture was stirred continuously at room temperature. After two weeks of incubation, the cells were thoroughly dialyzed against PBS, and the total amount of peptides recovered was 90%.