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利用培养大鼠视网膜色素上皮（ＲＰＥ）细胞，我们观察了多种受体激动剂／拮抗剂对ＲＰＥ细胞内第二信使磷酸肌醇（ＩｎｓＰｓ）水平的影响。结果表明，５－羟色胺（５－ＨＴ）及５－ＨＴ２受体激动剂如α－甲基－５－羟色胺、Ｑｕｉｐａｚｉｎｅ、和ＤＯＩ（１－［２．５ｄｉｍｅｔｈｏｘｙ－４－ｉｏｄｏｐｈｅｎｙｌ］－２－ａｍｉｎｏｐｒｏｐａｎｃ）均刺激大鼠ＲＰＥ细胞［３Ｈ］－ＩｎｓＰｓ形成，５－ＨＴ刺激［３Ｈ］－Ｉｎｓｐｓ形成的效应可被５－ＨＴ２受体拮抗剂Ｋｅｔａｎｓｅｒｉｎ完全阻断，而５－ＨＴ３，拮抗剂ＭＤＬ７２２２２对之无阻断作用。５－ＨＴ的效应还可被蛋白激酶Ｃ激活剂ＰＭＡ（４β－ｐｈｏｒｂｏｌ１２－ｍｙｒｉｓｔａｔｃ１３－ａｃｅｔａｔｅ）部分抑制。结果清晰表明，在大鼠ＲＰＥ细胞存在着与Ｉｎｓｐｓ信使通路相偶联的５－ＨＴ２受体。
Using cultured rat retinal pigment epithelium (RPE) cells, we examined the effect of various receptor agonists / antagonists on the second messengers of phosphoinositides (InsPs) in RPE cells. The results showed that serotonin (5-HT) and 5-HT2 receptor agonists such as α-methyl-5-hydroxytryptamine, Quipazine, and DOI (1- [2.5dimethoxy-4-iodophenyl] -2-aminopropanc) Stimulated the formation of [3H] -InsPs in rat RPE cells. The effect of 5-HT stimulation on the formation of [3H] -Insps was completely blocked by Ketanserin, a 5-HT2 receptor antagonist, but not by 5-HT3, antagonist MDL72222 Blocking effect. The effect of 5-HT is also partially inhibited by the protein kinase C activator PMA (4β-phorbol12-myristatc13-acetate). The results clearly show that there is a 5-HT2 receptor coupled to the Insps messenger pathway in rat RPE cells.