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目的利用蛋白芯片技术检测高原红细胞增多症(HAPC)患者与高原正常人血清炎症细胞因子的表达,探讨血清炎症细胞因子在HAPC发病中的作用及可能的机制。方法选择与慢性高原病密切相关的40种炎症细胞因子作为可能影响HAPC发病的检测指标,应用低密度蛋白质芯片检测8名HAPC患者及9名高原正常人的血清标本,并利用SRING9.1数据库做蛋白质相互作用分析。结果 HAPC患者与高原正常人的血清炎症细胞因子蛋白表达水平:IL-1β为488.92±91.78 vs 226.65±127.62、IL-2为590.64(707.02-460.88)vs 280.79(294.70-205.27)、IL-3为711.38±197.51 vs 271.64±135.69、IL-15为534.50±143.04 vs 367.98±108.10、IL-16为655.55±150.86 vs394.75±110.74、MCP-1为2304.70(3 901.71-1 928.07)vs 1421.61(2 099.36-1241.21)、TNF-α为591.43±103.03 vs 433.18±141.12(P<0.05);其余33种炎症细胞因子变化甚小(P>0.05)。结论 IL-1β、IL-2、IL-3、IL-15、IL-16、MCP-1、TNF-α可能均参与了HAPC的发病过程,且与EPO、HIF-1α、雄激素受体(AR)有内在的相互关联作用;蛋白芯片技术对于探讨HAPC发病机制及开发快速准确临床检验是技术1种具有实用性和有效性的工具。
Objective To detect the expression of serum inflammatory cytokines in patients with high altitude polycythemia (HAPC) and normal high altitude patients by protein microarray, and to explore the possible role of serum inflammatory cytokines in the pathogenesis of HAPC. Methods 40 kinds of inflammatory cytokines closely related to chronic high altitude sickness were selected as the possible indicators of HAPC incidence. Serum samples of 8 HAPC patients and 9 healthy people were detected by low-density protein chip and analyzed by SRING9.1 database Protein interaction analysis. Results Serum levels of inflammatory cytokines in patients with HAPC and normal altitude were 488.92 ± 91.78 vs 226.65 ± 127.62, IL-2 was 590.64 (707.02-460.88) vs 280.79 (294.70-205.27), IL-3 was 711.38 ± 197.51 vs 271.64 ± 135.69, IL-15 was 534.50 ± 143.04 vs 367.98 ± 108.10, IL-16 was 655.55 ± 150.86 vs 394.75 ± 110.74, MCP-1 was 2304.70 (3 901.71-1 928.07) vs 1421.61 (2 099.36 -1241.21). The TNF-α was 591.43 ± 103.03 vs 433.18 ± 141.12 (P <0.05). The other 33 inflammatory cytokines changed little (P> 0.05). Conclusion IL-1β, IL-2, IL-3, IL-15, IL-16, MCP-1 and TNF-α may all participate in the pathogenesis of HAPC, AR) are intrinsically interrelated; protein chip technology for the study of HAPC pathogenesis and development of rapid and accurate clinical test is a technique of practicality and effectiveness of the tool.