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目的Z-HL16C建株后对其性质和应用的研究。方法将该细胞传代冻存再复苏、扩大应用,进而对其性质进行鉴定。结果类上皮细胞,形态均一、界限清晰、有立体感。经传代、培养、冻存至今已10年,复苏率90%以上。该细胞株对脊髓灰质炎,柯萨奇A-B,艾柯病毒,甲、乙型流感病毒,1~3型副流感病毒,合胞病毒,麻疹病毒,风疹病毒,腺病毒(2,3,7,11型),1~2型单纯疱疹病毒,水痘带状疱疹病毒及巨细胞病毒均敏感,经鉴定为癌变细胞系。结论Z-HL16C为稳定的、对病毒广谱敏感的癌变细胞系。
Objective Z-HL16C after the establishment of its nature and application. Methods The cells were cryopreserved and then resuscitated, expanded for further application, and their properties were identified. Results of epithelial cells, uniform shape, clear boundaries, a sense of three-dimensional. After passage, training, frozen has been 10 years, the recovery rate of 90%. This cell strain has been proven to be effective against poliomyelitis, coxsackie AB, echovirus, influenza virus type A, influenza B, parainfluenza virus type 1 to 3, syncytial virus, measles virus, rubella virus, adenovirus , 11), herpes simplex virus type 1 ~ 2, varicella-zoster virus and cytomegalovirus were sensitive, identified as a cancerous cell line. Conclusion Z-HL16C is a stable, broad-spectrum virus-sensitive cancer cell line.