目的研究嗅鞘细胞(OECs)和神经干细胞(NSCs)分别及共培养的相互作用,为CNS神经损伤修复提供移植的理论依据。方法分离培养NSCs和OECs,在无血清DMEM/F12液中NSCs和OECs共培养,用免疫细胞化学和激光共聚焦显微术来分析结果。结果共培养7d观察OECs能促进NSCs增殖分化,分化为神经元、星形胶质细胞和少突胶质细胞,其中神经元比例高达78.2%。结论OECs可促进NSCs的增殖分化,其分化的神经元比例高。 OBJECTIVE: To study the interaction between OECs and neural stem cells (NSCs) and co-culture respectively and provide the theoretical basis for the transplantation of CNS injury. Methods NSCs and OECs were isolated and cultured. NSCs and OECs were co-cultured in serum-free DMEM / F12 solution. Immunocytochemistry and confocal laser scanning microscopy were used to analyze the results. Results After cultured for 7 days, OECs could promote the proliferation and differentiation of NSCs and differentiate into neurons, astrocytes and oligodendrocytes. The proportion of neurons was 78.2%. Conclusion OECs can promote the proliferation and differentiation of NSCs, and the proportion of differentiated neurons is high.