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不同棉花品种,茎基针刺接种大丽轮枝菌中等毒力小种(SS—4)或强毒力小种(T—1)3日内,经室内培养,自棉茎端部维管束和叶柄组织分离到该病原菌.在所有供试的感耐,抗病品种温室培养的幼苗中均观察到大丽轮枝菌在棉茎中的这种传导。7—9日后叶部显现症状。自抗病品种SBSl 上分离到病原菌的频率低于自耐、感病陆地棉品种分离到的频率。在茎段上接种萌发单孢,抗病品种棉茎段比之感、耐病品种茎段能更强烈抑制大丽轮枝菌菌丝的生长。在抗病品种茎干切段上,即使病菌能够生长,产生的微菌核也少,很晚。而感病品种切段上,菌丝生长旺盛,产生的微菌核也多。接种后不久,抗、感病品种棉茎端部和叶柄组织中大丽轮枝菌的存在,及抗、感病品种段上菌丝生长和微菌核形成的差异表明,这种抗性是一种被激活的寄主化学反应。这些结果与下一篇关于类倍半萜烯植保素的积累的论文中所报道的资料相一致。这种植保素可能是棉花抗黄萎病的关键因子。
Different cotton cultivars and stem-based needles were used to inoculate three strains (SS-4 or T-1) of Verticillium dahliae medium-density needle (T-1) Petiole tissue was isolated from this pathogen.This conduction of V. dahliae in cotton stems was observed in all of the seedlings of greenhouse cultivars tested in susceptible and resistant varieties. 7-9 days after the emergence of the symptoms of the leaves. The frequency of pathogenic bacteria isolated from SBSl was lower than that of self-tolerance and susceptible upland cotton cultivars. Inoculation of single spore on the stem segment, resistant varieties of cotton stem segment than the sense of disease-resistant varieties of stems can be more strongly inhibited the growth of Verticillium dahliae mycelium. In the stem varieties of disease-resistant varieties cut, even if the bacteria can grow, the generation of micro-sclerotia also less, very late. The susceptible varieties cut into sections, vigorous growth of mycelium, micro-sclerotia also produce more. Shortly after inoculation, the presence of Verticillium dahliae in the ends and petiole tissues of the resistant and susceptible cultivars, and the differences in mycelial growth and mycobacterial formation on the resistant and susceptible cultivars showed that this resistance was An activated host chemical reaction. These results are consistent with the data reported in the next paper on the accumulation of sesquiterpene phytoalexins. This phytoalexin may be a key factor in cotton’s resistance to Verticillium wilt.