利多卡因在pH3.4时能与金莲橙OOⅣ结合成复合物并溶于氯仿中,将提取的复合物还原,测还原金莲橙燐光,由此计算利多卡因的含量。本法可检测3ng利多卡因,用于血中利多卡因测定,重复性较好,可定量回收。 小鼠im(10mg/kg),狗iv(4mg/kg)利多卡因后,测血药浓度,经药物动力学分析为二室型,其血药浓度表达式为:小鼠:C_((μg/ml))=2.49e~(-0.00677t)+3.43e~(-0.0217t -7.14e~(-0.0892t)。犬:C((μg/ml))=2.03e~(-0.0142t)+2.68e~(-0.146t) Lidocaine at pH3.4 with the lotus orange OO Ⅳ combined into a complex and dissolved in chloroform, the extracted complex was reduced to measure the reduction of the orange lotus light, thus calculating the lidocaine content. This law can detect lidocaine 3ng, lidocaine for blood determination, better reproducibility, quantitative recovery. After the mice im (10mg / kg), dogs iv (4mg / kg) of lidocaine, the blood concentration was measured by pharmacokinetic analysis of two-compartment type, the plasma concentration is: mouse: C (C) (g / ml)) = 2.49e ~ (-0.00677t) + 3.43e ~ (-0.0217t -7.14e ~ (-0.0892t) ) + 2.68e ~ (-0.146t)