论文部分内容阅读
目的:探讨重组激肽释放酶结合蛋白(KBP)对胃癌淋巴管新生的抑制作用及其可能的分子机制。方法:培养SGC-7901胃癌细胞系,建立胃癌裸鼠异位移植瘤模型,了解KBP对胃癌生长和淋巴管新生的抑制作用。免疫组织化学和Western blot方法检测肿瘤细胞和组织中的血管内皮生长因子C(VEGF-C)表达,明确KBP通过减少肿瘤细胞VEGF-C表达抑制肿瘤淋巴管新生和肿瘤生长。结果:腹腔内注射KBP导致胃癌生长抑制,抑制率为61.4%。KBP处理的肿瘤组织中淋巴管密度显著降低,提示KBP可以抑制肿瘤淋巴管生成和肿瘤生长。免疫组织化学和Western blot检测显示KBP处理的SGC-7901胃癌细胞和组织中VEGF-C的表达显著减少。结论:下调VEGF-C在肿瘤细胞和肿瘤组织中的表达,提示KBP可能通过抑制肿瘤淋巴管新生作用,进而抑制肿瘤淋巴转移的作用。
Objective: To investigate the inhibitory effect of recombinant kallikrein binding protein (KBP) on lymphangiogenesis in gastric cancer and its possible molecular mechanism. Methods: SGC-7901 gastric cancer cell lines were cultured and established xenograft model of gastric cancer in nude mice to understand the inhibitory effect of KBP on gastric cancer growth and lymphangiogenesis. Immunohistochemistry and Western blot were used to detect the expression of vascular endothelial growth factor C (VEGF-C) in tumor cells and tissues. It was found that KBP inhibited tumor lymphangiogenesis and tumor growth by reducing the expression of VEGF-C in tumor cells. Results: Intraperitoneal injection of KBP resulted in the growth inhibition of gastric cancer with the inhibition rate of 61.4%. Lymphatic vessel density was significantly reduced in KBP-treated tumor tissue, suggesting that KBP can inhibit tumor lymphangiogenesis and tumor growth. Immunohistochemistry and Western blot showed that the expression of VEGF-C was significantly decreased in KBP-treated SGC-7901 gastric cancer cells and tissues. Conclusion: The down-regulation of VEGF-C expression in tumor cells and tumor tissues suggests that KBP may inhibit tumor lymphatic metastasis by inhibiting tumor lymphangiogenesis.