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采用改进的乳酸 - PAGE技术对不同玉米 (Zea mays)自交系和杂交种种子的白蛋白和球蛋白进行了系统电泳分析 ,发现此方法具有高度的分辨力和稳定性。把电泳图依其迁移率分为 α、β、γ和 ω4个带区。各自交系和杂交种的电泳图均有其鲜明特征 ,彼此可以区别 ,视作它们各自的“指纹”。全籽粒电泳谱带与胚的电泳谱带基本相同。胚乳产生谱带少而弱 ,且大都和胚的谱带发生重叠。亲本自交系的不同谱带在杂种 F1 发生互补。杂种谱带等于两亲本共同谱带与各自独有谱带之和。而且正、反交 F1 谱带以及它们两亲本种子提取液机械混合产生的谱带完全相同 ,表明谱带的差异是一种由核基因控制的遗传性状。F1 的谱带类型可由两亲本谱带类型预测 ,也可由两亲本种子提取液混合而产生的电泳图所演示
By using the improved lactic acid - PAGE technique, the albumin and globulin of different maize (Zea mays) inbred lines and hybrid seeds were systematically analyzed by electrophoresis and found that this method has high resolution and stability. The electrophoresis according to their mobility is divided into α, β, γ and ω4 band. Electrophoresis patterns of the respective crossbreds and hybrids have distinctive features that distinguish them from one another as their respective “fingerprints.” Whole grain electrophoresis bands and embryos electrophoresis bands are basically the same. The endosperm produces fewer and weaker bands, and most of them overlap with the embryo’s band. The different bands of the parental inbred lines are complementary to F1 hybrids. The hybrid band is equal to the sum of the common band of the amphipathic and its own unique band. Moreover, the positive and negative cross F1 bands and their amphiphilic seed extracts produced the same bands mechanically, indicating that the band gap is a genetic trait controlled by the nuclear gene. The band type for F1 can be predicted from the amphipathic band type or from the electrophoretic pattern resulting from the mixture of amphipathic seed extracts