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虽然公认用Protein A-Sepharose亲和层析柱进行亲和层析是纯化IgG的最好的办法,但由于ProteinA和IgG_1的亲和性特别低,因此,IgG_1的纯化依然是一个难题。 我们用Bio-Rad公司的新产品Affi-Gel ProteinA MAPS Ⅱ kit从腹水中纯化小鼠IgG_1单克隆抗体
Although affinity chromatography with Protein A-Sepharose affinity chromatography is generally considered as the best method for purifying IgG, the purification of IgG_1 is still a problem due to the extremely low affinity of ProteinA and IgG_1. We purified the mouse IgG_1 monoclonal antibody from ascites using Bio-Rad’s new Affi-Gel ProteinA MAPS II kit