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目的探讨组织蛋白酶B(CB)在膀胱移行细胞癌(TCC)中的表达以及其与TCC浸润的关系。方法取TCC标本40例,TCC分级Ⅰ级23例,Ⅱ～Ⅲ级17例;表浅型TCC(Tis,Ta,T1期)25例,浸润型TCC(T2～4期)15例。另取10例正常膀胱组织作为对照。用链霉素抗生物素蛋白-过氧化物酶连接法行CB免疫组织化学染色观察并计算CB阳性细胞百分率。结果正常膀胱组织中基质无明显着色;在TCC癌组织中,CB可为细胞染色,部分基质亦有染色,部分毛细血管内皮细胞及部分成纤维细胞CB表达阳性,在癌周血管内皮细胞的阳性着色CB表达增强。CB在分级和分期高的癌组织中多为弥散阳性染色。TCC分级Ⅰ级组、TCC分级Ⅱ～Ⅲ级组、TCC分期表浅型组、TCC分期浸润型组及正常对照组的CB阳性细胞百分比分别为10.53%±3.76%、21.52%±3.58%、11.32%±2.69%、20.57%±3.25%、0.11%±0.18%,TCC各组均高于正常对照组(P<0.01);TCC分级Ⅱ～Ⅲ级组高于TCC分级Ⅰ级组,TCC分期浸润型组高于TCC分期表浅型组,差异均有统计学意义(P<0.01)。结论 CB可能成为判断TCC进展和预后的重要指标。
Objective To investigate the expression of cathepsin B (CB) in bladder transitional cell carcinoma (TCC) and its relationship with infiltration of TCC. Methods TCC specimens were collected from 40 patients with TCC grade Ⅰ, 23 patients with grade Ⅰ, 17 patients with grade Ⅱ ~ Ⅲ. TCC (25 Tis, Ta, T1) and 15 infiltrating TCC (T2 ~ 4) were obtained. Another 10 cases of normal bladder tissue as a control. The percentage of CB positive cells was observed by CB immunohistochemical staining with streptavidin-peroxidase ligation. Results The normal bladder tissue showed no obvious staining. In TCC tissues, CB could be stained by cells, some of the stroma was also stained, some of the capillary endothelial cells and some of the fibroblasts were positive in CB. Coloring CB expression increased. CB in the grading and staging cancer tissue mostly diffuse positive staining. The percentage of CB positive cells in TCC grade Ⅰ group, TCC grade Ⅱ ~ Ⅲ grade group, TCC staging superficial group, TCC staging infiltration group and normal control group were 10.53% ± 3.76%, 21.52% ± 3.58%, 11.32 TCC grade Ⅱ ~ Ⅲ group was higher than TCC grade Ⅰ group, TCC staging infiltration (P <0.01) Type group was higher than the TCC staged superficial group, the differences were statistically significant (P <0.01). Conclusion CB may be an important index to judge the progress and prognosis of TCC.