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目的 探讨准分子激光角膜切削术 (PRK)后肿瘤坏死因子 α(TNF- α)的表达及其对角膜创伤修复的作用。对比 10 g· L- 1 强地松龙和 1g· L- 1 双氯芬酸钠对 TNF- α表达的影响。方法 应用半定量反转录 -多聚酶链式反应 (semi- quantitative,RT- PCR)的方法探测 PRK后兔角膜细胞活性因子不同时间 m RNA表达的变化 ,并在相应时间点观察细胞学改变。结果 TNF-α在 PRK后 m RNA表达随时间变化 ,表达水平有改变 ,高峰出现在术后 2 4h。10 g· L- 1强地松龙完全抑制 TNF- α的表达 ,双氯芬酸钠无显著影响。结论 在高能量短暂作用的紫外光照射下角膜组织选择性地表达 TNF-α,提示这一细胞因子在PRK后角膜创伤修复过程中有一定作用 ,并证实 10 g· L- 1强地松龙能在转录水平抑制TNF- α的表达
Objective To investigate the expression of tumor necrosis factor α (TNF-α) and its effect on the repair of corneal wound after excimer laser keratectomy (PRK). The effect of 10 g · L-1 prednisolone and 1 g · L-1 diclofenac sodium on TNF-α expression was compared. Methods Semi-quantitative reverse transcription-polymerase chain reaction (semi-quantitative, RT-PCR) method was used to detect the expression of m RNA in rabbit corneal cells after different doses of PRK. The cytological changes were observed at the corresponding time points. Results The mRNA expression of TNF-α changed with time after PRK, the expression level changed, and the peak appeared at 24 h after operation. 10 g · L -1 prednisolone completely inhibited the expression of TNF-α, diclofenac sodium had no significant effect. Conclusions The selective expression of TNF-α in corneal tissue under high-energy short-term ultraviolet light irradiation suggests that this cytokine may play a role in corneal wound repair after PRK, and confirmed that 10 g · L -1 prednisolone Can inhibit the expression of TNF-α at the transcriptional level