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目的:探讨血清IgG型抗内皮细胞抗体(AECA)的阳性表达及其在不同分型糖尿病视网膜病变(DR)间的变化趋势。方法:根据荧光素眼底血管造影(FFA)将糖尿病视网膜病变患者分为增殖型糖尿病视网膜病变(PDR)组、背景型糖尿病视网膜病变(BDR)组、糖尿病无视网膜病变(NDR)组及正常对照组。用体外培养的人脐静脉内皮细胞(HUVEC)作为抗原,采用间接免疫荧光(IIF)法检测不同分型患者血清IgG型抗内皮细胞抗体阳性率的变化,并与正常对照组进行对比。结果:血清IgG型AECA阳性表达BDR组和PDR组均高于NDR组和正常对照组(P<0.05),而BDR组和PDR组间阳性率差别无统计学意义(P>0.05),NDR组和正常对照组间阳性率差别无统计学意义(P>0.05)。结论:随着由正常眼底向DR的转变,血清IgG型AECA阳性率呈增高趋势,表明AECA与DR存在内在关联。
Objective: To investigate the positive expression of serum IgG anti-endothelial cell antibody (AECA) and its change trend in different type diabetic retinopathy (DR). Methods: Patients with diabetic retinopathy were divided into proliferative diabetic retinopathy (PDR) group, background diabetic retinopathy (BDR) group, diabetic retinopathy (NDR group) and normal control group according to fluorescein fundus angiography (FFA) . HUVECs were used as antigen to detect the positive rate of IgG anti-endothelial antibody in different types of patients by indirect immunofluorescence (IIF) and compared with the normal control group. Results: Serum IgG-positive AECA expression in BDR group and PDR group was significantly higher than that in NDR group and normal control group (P <0.05), but there was no significant difference between BDR group and PDR group (P> 0.05) There was no significant difference between the positive control group and normal control group (P> 0.05). CONCLUSIONS: The positive rate of serum IgG AECA is increasing with the transition from normal fundus to DR, indicating that AECA is intrinsically associated with DR.