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目的：建立稳定的大鼠脑出血模型，研究强啡肽Ａ１～１３（ＤｙｎＡ１～１３）对大鼠胶原酶诱导的脑出血后脑水肿的影响，寻找治疗脑出血后脑水肿的特异性药物。方法：脑立体定位下，大鼠大脑尾核内微量注射０．４ＩＵ细菌胶原酶；一般（ＨＥ）与神经元特殊（尼氏）染色后观察脑出血后病理改变；干湿质量法测定脑组织含水量。结果：大鼠尾核注射胶原酶可以造成稳定的大鼠脑实质出血，２４ｈ可形成脑血肿，其大小与注射剂量有关，脑出血后脑组织含水量与注射胶原酶的剂量呈正相关，ＤｙｎＡ１～１３可以减轻胶原酶诱导的脑出血后脑水肿，并与剂量有关。结论：脑实质内注射胶原酶可制备大鼠脑出血模型，ＤｙｎＡ１～１３对脑出血后脑水肿可能有一定的治疗作用。
Objective: To establish a stable intracerebral hemorrhage rat model and study the effect of DynA1 ~ 13 on cerebral edema after intracerebral hemorrhage induced by collagenase in rats, in order to find a specific drug for cerebral edema after intracerebral hemorrhage. Methods: Under stereotaxic conditions, microinjection of 0.4 IU bacterial collagenase into the caudate nucleus of rat brain was performed. The pathological changes of cerebral hemorrhage were observed after HE staining and neuronal staining (Nissl staining) Water content. Results: The injection of collagenase into rat caudate nucleus could cause stable cerebral parenchymal hemorrhage in rats. Cerebral hematoma could be formed in 24h and the size was related to injection dose. The content of water in brain tissue after intracerebral hemorrhage was positively correlated with the dose of collagenase injection. DynA1 ~ 13 Can reduce collagenase-induced cerebral edema after intracerebral hemorrhage, and dose-related. Conclusion: Intracerebral intracerebral injection of collagenase can prepare rat intracerebral hemorrhage model. DynA1 ~ 13 may have some therapeutic effect on cerebral edema after intracerebral hemorrhage.