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目的探讨局灶脑缺血再灌注损伤后神经细胞核因子-κB(NF-κB)和白细胞介素-6(IL-6)表达的变化。方法成年健康雄性Wistar大鼠32只,随机分为假手术组(n=4)和缺血再灌注组(n=28),应用线栓法制备大鼠脑缺血再灌注模型,免疫组织化学方法检测缺血再灌注6 h、12 h、1 d、2 d、3 d、7 d、14 d神经细胞NF-κB和IL-6的表达,并进行图像分析。结果假手术组大鼠脑组织神经细胞有微弱的NF-κB表达,阳性细胞为淡棕色。脑缺血再灌注6 h开始,皮质区和纹状体区神经细胞NF-κB表达逐渐增强,于再灌注1 d达高峰,之后逐渐下降。IL-6表达部位和变化规律与NF-κB相似,但其表达高峰在再灌注2 d。NF-κB和IL-6的表达水平在缺血再灌注损伤后的变化规律具有显著相关性(r=0.919 6,P<0.01)。结论NF-κB和IL-6表达在脑缺血再灌注损伤的病理过程中起重要作用。
Objective To investigate the changes of the expression of nuclear factor-κB (NF-κB) and interleukin-6 (IL-6) after focal cerebral ischemia-reperfusion in rats. Methods Thirty-two adult male Wistar rats were randomly divided into sham operation group (n = 4) and ischemia reperfusion group (n = 28). The cerebral ischemia-reperfusion model was established by thread occlusion. Immunohistochemistry Methods The expression of NF-κB and IL-6 in nerve cells at 6 h, 12 h, 1 d, 2 d, 3 d, 7 d and 14 d after ischemia-reperfusion were detected and analyzed by image analysis. Results The sham-operated rats showed weak expression of NF-κB in brain cells and light brown cells. Six hours after cerebral ischemia-reperfusion, the expression of NF-κB in cortical and striatum neurons gradually increased and peaked on the first day of reperfusion, then decreased gradually. The expression of IL-6 was similar to that of NF-κB, but the peak of expression was at 2 days after reperfusion. There was a significant correlation between the expression of NF-κB and IL-6 after ischemia-reperfusion (r = 0.919 6, P <0.01). Conclusion The expressions of NF-κB and IL-6 play an important role in the pathological process of cerebral ischemia-reperfusion injury.