论文部分内容阅读
目的 观察氧化苦参碱预防大鼠肝纤维化的疗效并探讨其作用机制。方法 建立半乳糖胺和二甲基亚硝胺大鼠肝纤维化模型 ,观察氧化苦参碱 (3 0mg/kg、90mg/kg)预防前后肝指数、血及肝组织生化、羟脯氨酸含量、TGFβ1mRNA表达、电镜及病理组织学改变。结果 氧化苦参碱预防组较模型组肝功能改善、肝组织羟脯氨酸含量及TGFβ1表达水平降低 (P <0 .0 1) ;预防组肝组织内SOD、GST PX较模型组升高 ,而MDA低于模型组 ;电镜显示肝细胞损伤减轻 ,病理组织学明显改善。结论 氧化苦参碱对半乳糖胺及二甲基亚硝胺诱导的肝纤维化均有预防作用 ,其主要机制为抗脂质过氧化、保护肝细胞和抑制肝星状细胞活化 ,从而抑制纤维生成等
Objective To observe the curative effect of oxymatrine on preventing hepatic fibrosis in rats and its mechanism. Methods The model of hepatic fibrosis induced by galactosamine and dimethylnitrosamine in rats was established. The liver index, blood and liver biochemical, hydroxyproline content before and after the prevention of oxymatrine (30 mg / kg, 90 mg / kg) , TGFβ1 mRNA expression, electron microscopy and histopathological changes. Results Compared with the model group, the oxymatrine prevention group improved the hepatic function, the content of hydroxyproline and the expression of TGFβ1 in liver tissue decreased (P <0.01) While MDA was lower than the model group; electron microscopy showed that liver cell damage was alleviated and histopathology was significantly improved. Conclusion Oxymatrine can prevent hepatic fibrosis induced by galactosamine and dimethylnitrosamine. Its main mechanism is anti-lipid peroxidation, protecting liver cells and inhibiting the activation of hepatic stellate cells, thereby inhibiting fiber Generate and so on