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目的:探讨CD4+CD2+5调节性T细胞对糖尿病小鼠细胞活化的影响。方法:选取BALB/c小鼠,每日腹腔注射链脲佐菌素(STZ)40mg/kg,连续5天,建立1型糖尿病模型;从小鼠内眦静脉采血,检测血糖;取小鼠尿液,检测尿糖;流式细胞术检测BALB/c小鼠脾细胞悬液CD4+T细胞中CD28分子的表达水平及凋亡细胞的数量。结果:在注射STZ第2周和4周,BALB/c小鼠CD4+T细胞中的CD28水平未见明显变化;凋亡细胞的数量在注射STZ第2周明显升高(P<0.05),第4周进一步明显升高(P<0.01)。结论:CD4+CD25+调节性T细胞可能通过抑制CD28共刺激抑制BALB/c小鼠CD4+T细胞活化,从而影响细胞免疫功能。诱导免疫细胞凋亡可能是CD4+CD2+5调节性T细胞发挥抑制作用的又一表现形式。
Objective: To investigate the effect of CD4 + CD2 + 5 regulatory T cells on cell activation in diabetic mice. Methods: BALB / c mice were injected intraperitoneally with streptozotocin (STZ) 40mg / kg for 5 consecutive days to establish type 1 diabetes mellitus model. Blood was collected from the internal canthal vein of mice to detect blood glucose. Urine , Urine glucose was detected. The expression of CD28 in CD4 + T cells and the number of apoptotic cells in BALB / c mice spleen cell suspension were detected by flow cytometry. Results: There was no significant change in the level of CD28 in BALB / c mice at 2 weeks and 4 weeks after injection of STZ. The number of apoptotic cells was significantly increased at 2 weeks after STZ injection (P <0.05) The fourth week was significantly higher (P <0.01). CONCLUSION: CD4 + CD25 + regulatory T cells may inhibit the activation of CD4 + T cells in BALB / c mice by inhibiting CD28 costimulation, thereby affecting cellular immune function. Induction of immune cell apoptosis may be another manifestation of the inhibitory effect of CD4 + CD2 + 5 regulatory T cells.