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种植无病蕉苗是防治香蕉束顶病和花叶心腐病的根本措施。筛选无病蕉苗的快速测定技术。除可引用2,3,5-氯化三苯基四(氨)唑浸渍徒手切片;在36℃条件下。24小时后:束顶病株叶脉切片的维管束呈现红色,其它组织为红褐色;花叶心腐病株叶脉切片(含维管束)全部呈现黑褐色;而健株叶脉切片由原绿色变成红色,最后褪成无色。此外。也可取叶片主脉的徒手切片,用1％曙红B水溶液染色。健株切片呈桔红色,病株切片呈砖红色,通过镜检:束顶病株叶片切片的表皮下,可观察到围绕在维管束周围成堆的分布有畸形叶绿体细胞团;花叶心腐病的畸形叶绿体细胞团,多分布在薄壁细胞中,且多散生;而健株的切片没有发现这种畸形的叶绿体细胞团,两种方法同样可靠。
Planting diseased banana seedlings is the fundamental measure to prevent and cure banana bunchy top disease and mosaic heart rot disease. Screening of diseased banana seedlings rapid determination of technology. In addition to the reference 2,3,5-trimethylammonium chloride (ammonia) impregnated bare hands; 36 ℃ conditions. Twenty-four hours later, the vascular bundles of the leaf bundles in the top of the bunch were red and the other tissues were reddish-brown. The leaf segments (including vascular bundles) in the leaf turf were all dark brown. Red, finally faded colorless. In addition. It is also advisable to take a freehand section of the main vein of the leaf stained with 1% Eosin B in water. Healthy plant sections were orange, diseased plants showed a brick red, through the microscopic examination: Bunch top diseased leaves under the epidermis, can be observed around the vascular bundles around the piles distributed deformity chloroplast cell mass; Deformity of chloroplast cell mass, mostly in parenchyma cells, and more scattered; and healthy strains of the slice did not find this deformity chloroplast cell mass, the two methods are equally reliable.