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目前,线虫的固体培养方法是通过无菌操作技术,在动物组织匀浆(如鸡内脏匀浆)加海绵的培养基中加入共生细菌,接入线虫一级种而完成的(Bedding 1981,19841 Wouts 1981)。液体培养则是在接有共生细菌的液体培养基中加入线虫一级种而得到(Buecher et al.1989)。这类培养方法要求加入的线虫一级种仅携带其共生细菌才能达到单菌培养。如果线虫一级种带有杂菌,就会造成培养物的严重污染,大幅度降低线虫产量。因此建立线虫一级种的单菌培养方法对线虫的大量繁殖和工业化生产
At present, the nematode’s solid culture method is accomplished by asepsis technique, adding symbiotic bacteria into the culture medium of animal tissue homogenates (such as chicken internal organs homogenate) and sponge, and accessing the first grade of nematode (Bedding 1981,19841 Wouts 1981). Liquid culture is obtained by adding nematode species to liquid medium with symbiotic bacteria (Buecher et al., 1989). Such training methods require the addition of nematode species only carry their symbiotic bacteria in order to achieve a single culture. If the first species of nematode with bacteria, it will result in serious pollution of culture, significantly reduce the production of nematodes. Therefore, the establishment of a single species of nematode culture method of nematode mass reproduction and industrial production