[Objective] This experiment was conducted to compare the activity difference of digestive enzymes in Japanese eels Anguilla japonics and growth retarded eels under different reaction temperature.[Method] Both 20 normal Japanese eels(Weight from 126.4 to 140.2 g per eel) and 20 growth retarded eels(Weight from 3.5 to 8.6 g per eel) were selected,then,the protease,amylase,lipase activities in hepatopancreas,intestine and stomach under the reaction temperature 5,10,15,25,30,35,40,45 and 55℃ were measured.[Result] The protease activities in hepatopancreas,intestine and stomach all reached the highest value for both normal eels and growth retarded eels,and the order of protease activities was intestine > stomach > hepatopancreas except the protease activities of growth retarded eels at the temperature of 5,15,55℃.The protease activities in hepatopancreas,intestine and stomach of growth retarded eels were 29.5%,15.7% and 25.2% of those of normal eels(P<0.05).The amylase activities of both normal and growth retarded eels reached the highest value at the temperature of 30 ℃.The amylase activity order of normal eels was hepatopancreas > intestine > stomach expect the amylase activity at the temperature of 5 ℃,while the amylase activity order of growth retarded eels is intestine > hepatopancreas > stomach.The amylase activities in hepatopancreas,intestine and stomach of growth retarded eels were 42.4%,73.7% and 43.8% of those of normal eels(P<0.05).The lipase activities of both normal and growth retarded eels reached the highest value at the temperature of 35 ℃.The lipase activity order of normal eels was hepatopancreas > stomach > intestine,while the lipase activity order of growth retarded eels was hepatopancreas > intestine > stomach expect the lipase activity at the temperature of 35 ℃.The lipase activities in hepatopancreas,intestine and stomach of growth retarded eels were 41.5%,45.6% and 23.2% of those of normal eels(P<0.05).[Conclusion] The protease,amylase,lipase activities in hepatopancreas,intestine and stomach of growth retarded Japanese eels were significantly lower than those of normal Japanese eels,therefore,its growth was affected directly. [Objective] This experiment was conducted to compare the activity difference of digestive enzymes in Japanese eels Anguilla japonics and growth retarded eels under different reaction temperature. [Method] Both 20 normal Japanese eels (Weight from 126.4 to 140.2 g per eel) and 20 growth retarded eels (Weight from 3.5 to 8.6 g per eel) were selected, then, the protease, amylase, lipase activities in hepatopancreas, intestine and stomach under the reaction temperature 5,10,15,25,30,35,40,45 and 55 ℃ were measured. [Result] The protease activities in hepatopancreas, intestine and stomach all reached the highest value for both normal eels and growth retarded eels, and the order of protease activities was intestine> stomach> hepatopancreas except the protease activities of growth retarded eels at the temperature of 5, 15, 55 ° C. The protease activities in hepatopancreas, intestine and stomach of growth retarded eels were 29.5%, 15.7% and 25.2% of those of normal eels (P <0.05). The amylase activities of bot h normal and growth retarded eels reached the highest value at the temperature of 30 ° C. The amylase activity order of normal eels was hepatopancreas> intestine> stomach expect the amylase activity at the temperature of 5 ° C while while the amylase activity order of growth retarded eels is intestine> hepatopancreas> stomach. The amylase activities in hepatopancreas, intestine and stomach of growth retarded eels were 42.4%, 73.7% and 43.8% of those of normal eels (P <0.05). The lipase activities of both normal and growth retarded eels reached the highest value at the temperature of 35 ° C. The lipase activity order of normal eels was hepatopancreas> stomach> intestine, while the lipase activity order of growth retarded eels was hepatopancreas> intestine> stomach expect the lipase activity at the temperature of 35 ° C. The lipase activities in hepatopancreas, intestine and stomach of growth retarded eels were 41.5%, 45.6% and 23.2% of those of normal eels (P <0.05). [Conclusion] The protease, amylas e, lipase activities in hepatopancreas, intestine and stomach of growth retarded Japanese eels were significantly lower than those of normal Japanese eels, therefore, its growth was affected directly.