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目的:探讨哮喘患者外周血调节性T细胞(Treg)以及辅助性T细胞(Th1/Th2)的比例的变化,探讨其在哮喘的临床治疗中的作用。方法:80例哮喘患者(哮喘组)按临床表现分为急性发作期组(54例)和缓解期组(26例),同时选择50例健康体检者。应用流式细胞仪检测上述各组外周血CD4+CD25+Foxp3+Treg、CD4+IFN-γ+Th1和CD4+IL-4+Th2细胞水平,并进行统计学分析。结果:哮喘组CD4+CD25+Foxp3+Treg水平亦明显低于正常对照组(P<0.05。其中急性发作期组Treg水平明显低于缓解期组和正常对照组(P<0.05)。而哮喘组Th1/Th2比值显著低于对照组(P<0.05),且在哮喘急性发作组中Th1/Th2比值显著低于缓解期组和正常对照组(P<0.05)。结论:提示Treg和Th在哮喘的发生和发展中起着重要的作用。
Objective: To investigate the changes of the ratio of Treg and T helper (Th1 / Th2) in peripheral blood of asthmatic patients, and to explore its role in the clinical treatment of asthma. Methods: Eighty asthmatic patients (asthma group) were divided into acute exacerbation group (n = 54) and remission group (n = 26) according to clinical manifestations. Fifty healthy subjects were also selected. The levels of CD4 + CD25 + Foxp3 + Treg, CD4 + IFN-γ + Th1 and CD4 + IL-4 + Th2 in the above groups were detected by flow cytometry and analyzed statistically. Results: The levels of CD4 + CD25 + Foxp3 + Treg in asthma group were also significantly lower than those in normal control group (P <0.05), while the level of Treg in acute phase was significantly lower than that in remission group and normal control group (P <0.05) (P <0.05), and Th1 / Th2 ratio in acute asthma attack group was significantly lower than that in remission group and normal control group (P <0.05) .Conclusion: It is suggested that Treg and Th in asthma The occurrence and development of the play an important role.