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为建立三黄痔疮膏中大黄素的质控标准。采用HPLC的方法进行含量测定 ,以乙醇为溶剂提取有效成分 ,去除基质。以甲醇 0 1%磷酸 (9∶1)为流动相 ,C18柱测定了大黄素的含量。结果测得三黄痔疮膏中大黄素含量为 0 0 6 6 3mg/g ,平均加样回收率 96 5 %。提示该法有效去除了栓剂基质对含量测定的影响 ,方法简便、准确、灵敏。
To establish quality control standards for emodin in Sanhuang Hemorrhoids Cream. The content was determined by HPLC, and the active ingredient was extracted with ethanol as the solvent to remove the matrix. Methanol 0 1% phosphoric acid (9:1) was used as the mobile phase and the emodin content was determined on a C18 column. The results showed that the content of emodin in Sanhuang Hemorrhoids cream was 0.666 mg/g, and the average recovery rate was 96.5%. It indicates that the method effectively removes the influence of suppository matrix on the content determination, and the method is simple, accurate, and sensitive.