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目的通过观察小鼠肠道优势菌群失衡肠黏膜上皮结构的变化以探讨肠道优势菌群失衡对黏膜机械屏障的影响。方法利用光镜及电镜技术观察轻度、重度菌群失衡小鼠肠道黏膜绒毛形态变化及上皮细胞超微结构的变化。结果菌群失衡小鼠肠黏膜绒毛出现肿胀、断裂,绒毛顶端肠上皮细胞坏死、脱落,重度菌群失衡与轻度菌群失衡小鼠比较绒毛结构受损加重。超微结构观察发现上皮细胞间隙增宽,胞浆内出现空泡结构,黏膜及黏膜下层有淋巴细胞浸润。结论抗生素干扰肠道优势菌群,可导致肠道机械屏障黏膜绒毛及超微结构受损且重度优势菌群失衡的损害大于轻度优势菌群失衡的损害。
Objective To observe the changes of intestinal epithelial imbalance in mice intestinal flora intestinal mucosal epithelial structure to explore the imbalance of intestinal flora on mucosal mechanical barrier. Methods The morphological changes of intestinal mucosa villi and the ultrastructure of epithelial cells in mice with mild and severe imbalances were observed by light microscopy and electron microscopy. Results Mice imbalance in the intestinal mucosa villi swelling, rupture, villus epithelial cell necrosis, shedding, severe imbalance of flora and mild imbalance of microflora in mice increased impairment. Ultrastructural observation found that the epithelial cell gap widened, cytoplasm vacuolization structure, mucosal and submucosal lymphocyte infiltration. Conclusion Antibiotics interfere with dominant intestinal microflora, which may lead to impaired mucosal villi and ultrastructure of intestinal mucosa, and the imbalance of severe dominant microflora is greater than that of mild dominant microflora.