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本实验利用免疫组化对胎儿心肌细胞增殖规律及细胞间质在发育中的变化进行了研究。先用医院水囊引产发育正常 3月龄～ 8月龄胎儿。采用 SP法对增殖细胞核抗原(PCNA) ,纤维粘连蛋白 (FN) , 胶原蛋白在心肌组织中的分布进行原位检测 ,用 DAB显色。胚胎期心肌组织均有PCNA阳性表达
In this study, we used immunohistochemistry to study the regulation of fetal cardiomyocytes proliferation and the development of interstitial cells. The first use of hospital water bag induced abortion normal 3 months to 8 months old fetus. The distribution of proliferating cell nuclear antigen (PCNA), fibronectin (FN) and collagen in myocardium was detected by SP method in situ and was visualized by DAB. Embryonic myocardial tissue PCNA positive expression