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目的:观察LDL-C边缘升高患者血清过氧亚硝酸阴离子(ONOO-)、脂联素、肿瘤坏死因子-α(TNF-α)水平的变化及意义。方法:选取LDL-C边缘升高患者50例和健康对照者40例,分别采用酶联免疫吸附法和放射免疫法测定血清ONOO-生成的标记物硝基酪氨酸(NT)、脂联素及TNF-α水平。结果:①与对照组比较,LDL-C边缘升高组血浆NT水平[(14.63±4.93)μmol/L∶(24.78±2.21)μmol/L,P<0.01]与脂联素水平[(5.17±2.36)μg/L∶(7.25±3.19)μg/L,P<0.01]增高,而TNF-α水平降低[(101.8±15.66)μg/L∶(50.37±16.31)μg/L,P<0.01]。②LDL-C边缘升高组血清LDL-C水平与脂联素水平(r=0.848 5,P<0.01)及NT水平(r=0.908 7,P<0.05)呈正相关,但与TNF-α无统计学相关性(P>0.05)。血清脂联素水平与TNF-α呈负相关(r=-0.539 4,P<0.01)。结论:LDL-C边缘升高患者血清ONOO-和脂联素增多,TNF-α的分泌下降。
OBJECTIVE: To investigate the changes of serum levels of ONOO-, adiponectin and tumor necrosis factor-α (TNF-α) in patients with elevated borderline LDL-C. Methods: Forty patients with borderline elevated LDL-C and 40 healthy controls were enrolled in this study. Enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay were used to detect the serum levels of ONOO-labeled nitrotyrosine (NT), adiponectin And TNF-α levels. Results: ① Compared with the control group, the level of plasma NT in the group of elevated LDL-C [(14.63 ± 4.93) μmol / L vs (24.78 ± 2.21) μmol / L, P <0.01] (P <0.01), and the level of TNF-α decreased ([101.8 ± 15.66] μg / L: (50.37 ± 16.31) μg / L, P <0.01) . ② Serum LDL-C level was positively correlated with adiponectin level (r = 0.848 5, P <0.01) and NT level (r = 0.908 7, P <0.05), but not with TNF-α Learning relevance (P> 0.05). Serum adiponectin levels were negatively correlated with TNF-α (r = -0.539 4, P <0.01). Conclusions: The serum levels of ONOO- and adiponectin increased and the secretion of TNF-α decreased in patients with elevated LDL-C margin.