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２０世纪８０年代初以来，戴乾圜根据其双区理论预言：辐射、石棉、异物植入等所谓物理致癌因子，也是通过诱发ＤＮＡ互补碱基股间交联而启动癌变．借碱稀释洗脱法证明， 石棉通过诱发细胞中氧化酶产生活化的过程，剂量相关地引发ＤＮＡ股间交联和ＤＮＡ－蛋白质间交联，其中ＤＮＡ股间交联达８０％以上．亚铁离子借Ｆｅｎｔｏｎ反应使交联率成倍地提高，维生素Ｃ则以因子８～９抑制交联，表明交联是由羟基自由基ＨＯ所诱发．非致癌剂芘或内源性致癌剂雌二醇分别与石棉共存时的不同交联增效作用，以及亚铁离子对两者的不同增效作用和维生素Ｃ的不同抑制作用，进一步证明交联是由ＨＯ所诱发．基于ＡＭ１计算指明了交联产物的可能结构，并借ＨＯ诱发的突变谱进一步证明了这些结构的存在．
Since the early 1980s, Dai Ganqi predicted based on its two-region theory that so-called physical carcinogenic factors such as radiation, asbestos, and foreign matter implantation also initiate canceration by inducing cross-linking between DNA complementary bases. Alkaline dilution elution method proved that asbestos induces the activation of oxidase in cells and induces cross-linking between DNA strands and DNA-protein cross-linking in a dose-dependent manner, in which DNA strand cross-linking is more than 80%. The ferrous ion by Fenton reaction doubled the rate of cross-linking, and vitamin C inhibited cross-linking with a factor of 8 to 9, indicating that the cross-linking was induced by hydroxyl radical HO. The cross-linking and synergistic effects of non-carcinogenic agents or endogenous carcinogens estradiol and asbestos, respectively, as well as different synergies of ferrous ions and different inhibitory effects of vitamin C, further prove cross-linking It was induced by HO. The possible structures of the cross-linked products were indicated on the basis of AM1 calculations, and the existence of these structures was further confirmed by the HO-induced mutation spectrum.