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目的:探讨草木犀的正丁醇提取物在炎症中的作用。方法:通过LPS干预RAW264.7细胞系建立炎症细胞模型,免疫细胞化学法检验NF-κB的表达及分布变化,实时荧光定量RT-PCR法检测血红素氧合酶1(HO-1)的基因表达,Western blot法测定HO-1的蛋白表达量。结果:免疫细胞化学法结果显示药物干预时胞质被染为棕色,NF-κB多分布在胞质未被激活;仅有LPS干预时,胞核被染为棕色,NF-κB集中分布在细胞核表达增强。药物提取物干预后HO-1的mRNA表达水平明显升高,且呈剂量依赖型关系;Western blot结果显示药物干预后HO-1的蛋白表达水平也明显升高。结论:草木犀的正丁醇提取物通过抑制NF-κB的激活,同时促进HO-1的释放而发挥一定的抗炎作用。
Objective: To explore the role of n-butanol extracts of Rhizoma sinica in inflammation. METHODS: The inflammatory cell model was established by the intervention of RAW264.7 cell line with LPS. The expression and distribution of NF-κB were detected by immunocytochemistry. The gene of heme oxygenase 1 (HO-1) was detected by real-time fluorescence quantitative RT-PCR. Expression, Western blot analysis of HO-1 protein expression. RESULTS: Immunocytochemistry results showed that the cytoplasm was stained brown during drug intervention, NF-κB was mostly not activated in the cytoplasm; only LPS intervention, the nucleus was stained brown, and NF-κB was concentrated in the nucleus. Enhanced expression. After treatment with drug extracts, the expression of HO-1 mRNA was significantly increased in a dose-dependent manner. Western blot showed that the protein level of HO-1 was significantly increased after drug intervention. Conclusion: The n-butanol extract of Rhizoma lycopersiculare exerts some anti-inflammatory effects by inhibiting the activation of NF-κB and simultaneously promoting the release of HO-1.