为探讨碱性成纤维细胞生长因子 (bFGF)对视网膜色素上皮 (RPE)细胞吞噬功能的影响。方法 :采用新生牛RPE细胞培养 ,并行免疫组化鉴定。对培养的RPE细胞于 0h、2 4h、48h 3个时间点分别给予0ng/ml、1ng/ml、4ng/ml、16ng/ml的bFGF后 ,加入鸡红细胞悬液 ,孵育 1h ,经清洗固定后于光镜下观察、计数 ,以判断RPE细胞吞噬功能。结果 :应用bFGF后 ,RPE细胞吞噬率及吞噬指数均明显增加 ,在一定范围内呈浓度及时间依赖性。结论 :bFGF对牛RPE细胞的非特异性吞噬功能有促进作用。 To investigate the effect of basic fibroblast growth factor (bFGF) on phagocytosis of retinal pigment epithelium (RPE) cells. Methods: Neonatal cattle RPE cells were cultured and identified by immunohistochemistry. The cultured RPE cells were treated with 0 ng / ml, 1 ng / ml, 4 ng / ml and 16 ng / ml bFGF respectively at 0 h, 2 4 h, and 48 h time points. In the light microscope observation, counting, in order to determine phagocytic function of RPE cells. Results: After application of bFGF, phagocytic rate and phagocytic index of RPE cells were significantly increased in a certain range of concentration and time-dependent manner. Conclusion: bFGF promotes the nonspecific phagocytosis of RPE cells in cattle.