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以甜瓜(Cucumis melo L.)抗蔓枯病自交系PI482398(含抗蔓枯病基因Gsb-4)和感病自交系‘白皮脆’以及它们的F1、BC1P1、BC1P2、F2群体为材料,苗期进行蔓枯病菌(Didymella bryoniae)接种鉴定,结果表明甜瓜抗蔓枯病基因Gsb-4为单显性遗传。利用集团分离分析法(bulked segregant analysis,BSA)对89对SSR引物进行筛选,引物CMTA170a在抗性材料中可扩增出约为120bp的条带,并与抗性基因Gsb-4表现出连锁关系。统计了CMTA170a在118个F2单株上的多态性,并利用MAPMAKER/Exp version3.0b软件进行了计算,其与Gsb-4的遗传连锁距离为5.14cM。
The population of F1, BC1P1, BC1P2, and F2 in resistant inbred lines PI482398 (including Gsb-4) and inbred lines susceptible to inbred lines (Cucumis melo L.) Materials and seedlings were inoculated with Didymella bryoniae. The results showed that Gsb-4, a resistance gene to melon, was a dominant dominant gene. 89 pairs of SSR primers were screened by bulked segregant analysis (BSA). The primer CMTA170a amplified a band of about 120 bp in the resistant material and showed a linkage relationship with the resistance gene Gsb-4 . The polymorphism of CMTA170a on 118 F2 plants was calculated and calculated using MAPMAKER / Exp version 3.0b software. The genetic linkage distance to Gsb-4 was 5.14cM.