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目的建立一种改良同步化无水乙醇乙酸染色体制备技术。方法将20份外周血标本同时使用两种方法制备染色体,对照组采用常规法;实验组采用无水乙醇替代甲醇配制固定液,同时使用胸苷、脱氧胞苷、高浓度秋水仙素同步化制备染色体,整个培养过程于试管中完成。结果两组相比,实验组获得的早期染色体分裂相数量较多,差异具有统计学意义(P<0.01);细胞分裂指数差异无统计学意义(P>0.05);染色体交叉重叠不多,分散良好,G显带清晰。结论实验组的改良技术步骤简单、操作简便,能获得较多带纹清晰的早期染色体,适合于临床常规应用。
Objective To establish a modified synchronized anhydrous ethanol acetic acid chromosome preparation technology. Methods Chromosomes were prepared from 20 peripheral blood samples by two methods at the same time. The control group was treated by conventional method. The experimental group was prepared with anhydrous ethanol instead of methanol to prepare the fixative solution. Simultaneously, thymidine, deoxycytidine and colchicine Chromosomes, the entire culture process in the test tube to complete. Results Compared with the two groups, the number of early chromosome cleavage phase in the experimental group was significantly higher than that in the control group (P <0.01). There was no significant difference in cell division index (P> 0.05) Good, G with clear. Conclusion The improved technique of the experimental group is simple and easy to operate, and can obtain more clear early chromosomes and is suitable for clinical routine application.