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建立了石墨烯修饰玻碳电极测定阿替洛尔的电化学分析新方法。采用电化学方法结合紫外、荧光光谱分析,研究了阿替洛尔(ATN)与牛血清白蛋白(BSA)的相互作用。实验发现,在pH 10.0的Na2CO3-NaHCO3缓冲溶液中,ATN在0.78 V处有一灵敏的氧化峰,氧化峰电流Ip与ATN的浓度在4.8～30.0μmol/L范围内呈良好的线性关系(r=0.9926,n=11),方法检出限为3μmol/L。当BSA加入ATN溶液后,ATN氧化峰电流降低,氧化峰电流的降低值ΔIp与BSA的浓度在2.6～31.0μmol/L范围内呈良好线性关系。紫外光谱表明ATN的加入使BSA的吸收峰发生蓝移。荧光光谱表明,ATN对BSA的内源荧光有显著的猝灭作用,猝灭机制为静态猝灭。
A new electrochemical method for the determination of atenolol by graphene modified glassy carbon electrode was established. The interaction between atenolol (ATN) and bovine serum albumin (BSA) was studied by electrochemical methods combined with ultraviolet and fluorescence spectroscopy. It was found that ATN had a sensitive oxidation peak at 0.78 V in Na2CO3-NaHCO3 buffer solution at pH 10.0. The peak current Ip showed a good linear relationship with ATN concentration in the range of 4.8-30.0 μmol / L (r = 0.9926, n = 11). The detection limit was 3μmol / L. When BSA was added into ATN solution, the oxidation peak current of ATN decreased. The decrease of oxidation peak current, ΔIp, was in good linear relationship with the concentration of BSA in the range of 2.6 ~ 31.0μmol / L. UV spectroscopy showed that the addition of ATN blue-shifted the absorption peak of BSA. Fluorescence spectra showed that ATN had a significant quenching effect on endogenous fluorescence of BSA and the quenching mechanism was static quenching.