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研究在不同骨关节炎中单克隆抗体(ED1)阳性细胞及降钙素基因相关肽(CGRP)阳性神经纤维在骨关节炎(OA)各个相同时期的表达变化及临床意义。将45只健康成年Wistar雄性大鼠随机分为正常组、手术组、跑步训练组,并进行OA造模。分别于1周、2周、3周、6周时处死大鼠,从各组对应时期取5只大鼠全膝关节组织置于4%PFA液固定、制片、免疫染色后,采用病理图像分析系统,通过观察及半定量ED1阳性细胞和CGRP阳性神经纤维的表达,评价两种骨关节炎中滑膜组织的早期病理变化。手术组及跑步训练组在1、2、3周较正常组均可见较多的ED1阳性细胞,手术组ED1阳性细胞升高明显,且手术组和跑步训练组在1、2、3周时ED1阳性细胞数呈极显著差异(P<0.01)。CGRP阳性神经纤维的表达显示:跑步训练组增多显著;2周时两组的CGRP阳性神经纤维数存在显著或极显著差异(P<0.05或0.01)。手术组的ED1阳性细胞的表达明显高于跑步训练组;而CGRP阳性神经纤维的表达显示跑步训练组高于手术组。
To investigate the changes and clinical significance of monoclonal antibody (ED1) positive cells and calcitonin gene related peptide (CGRP) positive nerve fibers in different stages of osteoarthritis at different stages of osteoarthritis (OA). Forty-five healthy adult Wistar male rats were randomly divided into normal group, operation group and running training group. Rats were sacrificed at 1 week, 2 weeks, 3 weeks and 6 weeks respectively. Totally 5 rats from each group were sacrificed and the whole knee joint tissues were fixed in 4% PFA solution. The analysis system was used to evaluate the early pathological changes of synovial tissue in two types of osteoarthritis by observing and semi-quantitating the expression of ED1-positive cells and CGRP-positive nerve fibers. The ED1-positive cells were more visible in the operation group and the running training group than those in the normal group at 1, 2, and 3 weeks. The ED1-positive cells in the operation group were significantly increased. ED1 The number of positive cells was significantly different (P <0.01). The expression of CGRP-positive nerve fibers showed that there was a significant increase in running training group. There was a significant or very significant difference in the number of CGRP-positive nerve fibers between the two groups at 2 weeks (P <0.05 or 0.01). The expression of ED1 positive cells in the operation group was significantly higher than that in the running training group. The expression of CGRP positive nerve fibers showed that the running training group was higher than the operation group.