大鼠骨髓源性耐受性树突状细胞的诱导培养

来源 :贵州医科大学学报 | 被引量 : 0次 | 上传用户:lovelywd
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目的探讨使用核因子-κB寡核苷酸诱骗剂(NF-κB ODN Decoy)诱导培养大鼠骨髓来源的耐受性树突状细胞(tol DC)的方法。方法取雄性Sprague Dawley(SD)和Wistar大鼠各10只,麻醉处死SD大鼠取胫骨制备骨髓细胞悬液、培养8 d获得树突状细胞(DC),分为Control-DC组[添加白细胞介素4(IL-4)和粒细胞-巨噬细胞集落刺激因子(GM-CSF)定向诱导分化培养]、Decoy-DC组(Control-DC组基础上加5μmol/L NF-κB ODN Decoy)、脂多糖
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