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目的 :研究神经生长因子 (NGF 2 .5S)对不同浓度的醋酸铅神经毒性拮抗效应 ,观察细胞分化以及细胞形态的影响 ,同时进行了醋酸铅的毒性试验和细胞分化的比较。方法 :利用大鼠胚胎中脑神经细胞作原代微团培养。观察不同浓度 ( 0 .0 0 1～ 5 0 μmol/L)醋酸铅对中脑细胞生长发育的影响。 结果 :染毒组的细胞集落数形成率明显降低 ,细胞体积小 ,细胞间神经纤维减少 ,表明醋酸铅可抑制胚胎中脑神经细胞的分化 ,其半数分化抑制浓度为 1 .0 μmol/L ,半数增殖抑制浓度为 2 0 .0 μmol/L ,并显剂量 效应关系。当加入NGF 2 .5S后 ,在同浓度的醋酸铅情况下 ,微团中的集落形成率有明显的增加。结论 :NGF 2 .5S对醋酸铅的神经毒性有一定的拮抗效应。
OBJECTIVE: To study the antagonistic effect of nerve growth factor (NGF 2 .5S) on the neurotoxicity induced by different concentrations of lead acetate, and to observe the effects of cell differentiation and cell morphology. At the same time, the toxicity of lead acetate and cell differentiation were compared. Methods: Rat embryonic mesencephalic neural cells were used as primary micelles. The effects of different concentrations of lead (0 0 1 ~ 500 μmol / L) on the growth and development of mesencephalic cells were observed. Results: The formation rate of colony was significantly decreased, the volume of cells was small and the number of intercellular nerve fibers was decreased, indicating that lead acetate could inhibit the differentiation of embryonic midbrain neurons. The half inhibitory concentration was 1.0 μmol / L, half Proliferation inhibition concentration was 2.0 μmol / L, and the dose-response relationship. When NGF 2 .5S was added, there was a significant increase in colony formation in micelles with the same concentration of lead acetate. Conclusion: NGF 2 .5S has certain antagonistic effect on the neurotoxicity of lead acetate.