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采用叶片针刺接种法从细胞学方面分析转hpa1xoo基因棉花与棉花黄萎病菌互作产生的微过敏抗病反应。通过细胞显微观察表明,转hpa1xoo基因棉花T-34与非转基因棉花在抗病性细胞表型方面存在明显差异。Trypan Blue染色后,伤口外的叶组织中均有一褐色环形条带,褐色环形条带与伤口坏死部位之间区域的组织细胞出现质壁分离和空腔细胞现象,褐色条带与伤口坏死部位之间的区域在转基因和非转基因棉花之间具有较明显的差异,转基因棉T-34较非转基因棉花品种Z35形成的区域小,说明转基因棉细胞损伤程度较非转基因棉细胞损伤程度低。棉花黄萎病菌侵染叶片后,非转基因棉花叶部伤口周围细胞无微过敏性反应(micro HR),受病原菌侵染的叶片萎蔫程度较重;转hpa1xoo基因棉花叶部伤口周围细胞有微过敏反应,叶部刺伤处仅有较小的坏死斑,且不相连,发病较轻,而清水处理的转基因棉花与非转基因棉花叶片中伤口周围均无微过敏反应,说明病原菌侵染诱导转hpa1xoo基因棉花产生微过敏防御反应,转hpa1xoo基因棉花较非转基因棉花有较强的抗病性。
The leaf needle acupuncture inoculation method was used to analyze the hypoallergenic disease resistance reaction of hpa1xoo transgenic cotton and Verticillium dahliae caused by cotton intercellular interaction. The results of cell microscopy showed that there was a significant difference in the phenotype of disease-resistant cells between transgenic cotton T-34 and non-transgenic cotton. Trypan Blue staining showed that there was a brown ring in the leaf tissue outside the wound, and the cells in the area between the brown ring and the wound necrosis appeared plasmolysis and cavity cell phenomenon. The brown bands and the necrotic sites There was obvious difference between the transgenic and non-transgenic cotton. The transgenic cotton T-34 had a smaller area than the non-transgenic cotton Z35, indicating that the damage degree of transgenic cotton cells was lower than the non-transgenic cotton cells. After the leaves were infected by Verticillium dahliae, the non-transgenic cotton leaves had no micro-hypersensitivity reaction (micro HR) and the wilts infected by the pathogen were more wilting. The cells around the hpa1xoo cotton leaflet were slightly hypersensitive Reaction, leaf stab at the lesion only small necrotic spots, and not connected, the incidence of light, and fresh water treatment of transgenic cotton leaves and non-transgenic cotton leaves without hypersensitivity reactions, indicating that the pathogen infection induced hpa1xoo Gene cotton micro-allergenic defense response, transgenic hpa1xoo cotton than non-transgenic cotton has a strong resistance.