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目的:观察金荞麦(Fagopyrumcymosum,Fag)提取物对肠易激综合征(irritablebowelsyndrome,IBS)样结肠刺激(colonirritation,CI)模型内脏高敏感性的改善作用以及对动物脊髓背角内5-HT及其受体的影响.方法:采用结肠刺激新生期乳大鼠来制作IBS样CI模型.CI大鼠成年后给予口服Fag2wk,并用腹壁撤退反射(AWR)评分来评价给药后CI大鼠内脏高敏感性的变化,取大鼠脊髓用免疫组织化学法观察5-HT免疫染色,用蛋白印迹法检测5-HT1A受体(5-HT1AR)、5-HT3A受体(5-HT3AR)表达.结果:和对照组比,CI组大鼠AWR评分明显增高(20mmHg:0.625±0.518vs1.333±0.778;30mmHg:0.750±0.463vs1.667±0.888;40mmHg:1.125±0.641vs2.000±0.739;50mmHg:1.500±0.926vs2.583±0.793;60mmHg:2.125±0.991vs3.083±0.669;均P<0.05);且脊髓内5-HT染色度增加(11.250±4.833vs21.125±7.827,P<0.01),5-HT3A受体表达升高(179.038±29.786,P<0.01),5-HT1A受体则表达降低(64.523±16.873,P<0.01);高剂量Fag可降低CI大鼠AWR评分(20mmHg:0.250±0.002;30mmHg:0.875±0.044;40mmHg:1.250±0.036;50mmHg:1.875±0.050;60mmHg:2.625±0.037;均P<0.05),并下调脊髓内5-HT(13.375±5.579,P<0.05)和5-HT3AR的表达(114.200±20.983,P<0.05),上调5-HT1AR的表达(93.008±13.523,P<0.05);低剂量Fag则对CI大鼠的影响不明显.结论:Fag对IBS样CI大鼠有镇痛作用,并通过调节其脊髓内5-HT及其受体来改善痛觉过敏.
OBJECTIVE: To observe the effect of Fagopyrumcymosum (Fag) extract on visceral hypersensitivity in irritable bowel syndrome (IBS) -like colon irritation (CI) model and the effects of 5-HT and And its receptor.Methods: IBS-like CI model was established by colonic stimulation of neonatal rats.CI rats were given oral Fag2wk orally, and their abdominal visceral reflex (AWR) scores were used to evaluate the visceral hypersensitivity 5-HT1A receptor (5-HT1AR) and 5-HT3A receptor (5-HT3AR) were detected by Western blotting.Results : Compared with the control group, the AWR score in CI group was significantly increased (20mmHg: 0.625 ± 0.518vs1.333 ± 0.778; 30mmHg: 0.750 ± 0.463vs1.667 ± 0.888; 40mmHg: 1.125 ± 0.641vs2.000 ± 0.739; 50mmHg: 1.500 ± 0.926vs2.583 ± 0.793; 60mmHg: 2.125 ± 0.991vs3.083 ± 0.669; all P <0.05). The 5-HT staining in the spinal cord increased (11.250 ± 4.833 vs 21.125 ± 7.827, P <0.01) The expression of 5-HT3A receptor was increased (179.038 ± 29.786, P <0.01), while the expression of 5-HT1A receptor was lower (64.523 ± 16.873, P <0.01) (20mmHg: 0.250 ± 0.002; 30mmHg: 0.875 ± 0.044; 40mmHg: 1.250 ± 0.036; 50mmHg: 1.875 ± 0.050; 60mmHg: 2.625 ± 0.037; all P < (114.00 ± 20.983, P <0.05), up-regulated the expression of 5-HT1AR (93.008 ± 13.523, P <0.05), while the effect of low dose Fag on CI rats was not significant.Conclusion : Fag has an analgesic effect on IBS-like CI rats and improves hyperalgesia by modulating 5-HT and its receptors in the spinal cord.