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目的 探讨施万细胞能否在体外促进神经干细胞的存活和分化。 方法 分离和克隆新生大鼠海马组织的神经干细胞 ;同时获取坐骨神经和臂丛神经 ,从中分离和纯化施万细胞。将施万细胞和神经干细胞进行共培养 ,借助免疫细胞化学技术检测培养的神经干细胞巢蛋白 (nestin)的表达及其分化后神经丝蛋白 (NF)和胶质原纤维酸性蛋白 (GFAP)的表达。应用扫描电镜观察神经干细胞的形态变化。 结果 与施万细胞一起培养的神经干细胞存活数量增加 ,分化为神经元样细胞的数量也明显增加。这些神经元样细胞的初级突起比对照组的明显增长。施万细胞能使神经干细胞胞体表面不规则的凹凸变得平整。共培养的施万细胞和神经干细胞可以 3种方式接触生长 :1 胞体与胞体接触 ;2 胞体与突起接触 ;3 突起与突起接触。 结论 施万细胞能促进体外培养的神经干细胞的存活及其分化为神经元样细胞
Objective To investigate whether Schwann cells can promote the survival and differentiation of neural stem cells in vitro. Methods The neural stem cells of hippocampus of neonatal rats were isolated and cloned. The sciatic nerve and brachial plexus nerves were obtained simultaneously, and Schwann cells were isolated and purified. The co-culture of Schwann cells and neural stem cells was performed. The expression of nestin in neural stem cells and the expression of neurofilament (NF) and glial fibrillary acidic protein (GFAP) after differentiation were detected by immunocytochemistry . Scanning electron microscopy was used to observe morphological changes of neural stem cells. Results The number of neural stem cells cultured with Schwann cells increased, and the number of differentiated neuron-like cells increased significantly. The primary neurites of these neuron-like cells showed a significant increase over the control. Schwann cells can make neural stem cell surface uneven irregularities become smooth. Co-cultured Schwann cells and neural stem cells may contact growth in three ways: 1 cell body and cell body contact; 2 cell body contact with the protrusion; 3 protrusion and protrusion contact. Conclusion Schwann cells can promote the survival of neural stem cells cultured in vitro and differentiate into neuron-like cells