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纳米粒子在生物医学上的应用越来越广泛,其进入细胞的机制与规律是设计与开发的基础.已有研究发现,表面修饰不同亲疏水性基团的金纳米粒子,在内吞机制被抑制时,进入细胞的能力明显不同.更特别的是,粒子表面亲水性基团与疏水性基团呈间隔条纹规则排列的纳米粒子,与其他修饰成分相同仅排列不同的纳米粒子进入细胞的规律区别显著.这一特殊现象无法用已有的纳米粒子进入细胞的机制解释.本文针对该研究结果,将纳米粒子与细胞的体系简化,定量分析了3种不同纳米粒子进入细胞前后的不同状态,计算获得了表面修饰不同亲疏水性基团的纳米粒子与细胞膜之间相互作用的Flory-Huggins自由能.结果发现,修饰规则间隔排列亲疏水基团的纳米粒子,其作用自由能在与细胞接触前后变化最大.研究结果不仅解释了实验发现,同时预示了纳米粒子进入细胞的新机制.
The application of nanoparticles in biomedicine is more and more widespread, and the mechanisms and rules of their entry into cells are the basis of design and development.It has been found that the surface modification of gold nanoparticles with different hydrophilic and hydrophobic groups is inhibited in endocytosis , The ability to enter cells is significantly different.More particularly, the particles on the surface of hydrophilic groups and hydrophobic groups arranged regularly spaced nanoparticles, and other modifications of the same arrangement of only different nanoparticles into the cell’s law The difference is significant.This particular phenomenon can not be explained by the mechanism that the existing nano-particles enter into the cell.According to the results of this study, the system of nano-particles and cells is simplified and the different states of three different nano- The Flory-Huggins free energy of the surface-modified nanoparticles with different hydrophilicity and hydrophobicity was calculated. The results showed that nanoparticles with hydrophobic groups arranged at regular intervals improved their free energy before and after contact with cells The biggest change.The results not only explain the experimental findings, but also indicates that the new mechanism of nanoparticles into the cell.